Nanoparticle Tracking Assay (NTA) is the most popular quantification method
In the new work, the POSTECH research groupLed by Dr. Xiwu Cho and Ph.D. Yohan Yi of the Mechanical Engineering Department, measured the amount, size and brightness of the scattered light through the NTA and further examined the fluorescence signals from individual particles.
The authors have developed a tracking analysis systemfluorescence-based nanoparticles (NTA) to understand the characteristics, size and protein expression of individual extracellular vesicles (EV). In this system, a sheet of lasers with four different wavelengths was directed to the EV in accordance with a specific graph: this resulted in a scattering image intercalated with three fluorescent images.
As a result, the authors were able to see the size, ratio and scattered light of thousands of individual particles.
The NTA system allows the user to monitornanoparticles during the analysis, authors can also obtain quantitative data on individual particles or all at once. This allows researchers to observe cells at the molecular level and will help them study various life processes in detail.
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