Non-invasive microscopic techniques such as optical coherence microscopy and two-photon microscopy are usually
Ballistic photons pass right throughobject, no deviation. They are used to restore the image of an object. In turn, multiple scattered photons are generated by random deflections as light passes through the material. As a result, they appear as grainy noise in the image. As the light travels further, the difference between scattered and ballistic photons increases, thus obscuring the information about the image.
Bone tissue has many complex internalstructures. They cause strong multiple scattering of light and complex optical aberrations. When it is necessary to obtain an optical image of the mouse brain through the intact skull, the fine structures of the nervous system are difficult to visualize. Grainy noise and other picture distortions interfere. This is a major obstacle in neurobiological research, where mice are often used as models. Because of such limitations in imaging techniques, the skull of mice must be removed or thinned out in order to examine the neural networks of the brain tissue beneath it.
A group of researchers led by a professorChoi Wonshika of the Center for Molecular Spectroscopy and Dynamics of the Institute for Basic Sciences (IBS) in Seoul, South Korea has made a major breakthrough in deep tissue optical imaging. They have developed a new optical microscope that can capture images through the intact skull of a mouse. As a result, scientists have access to a microscopic map of neural networks in brain tissues without loss of spatial resolution.
Schematic of a reflective array microscope developed by researchers at the IBS Center for Molecular Spectroscopy and Dynamics. Provided by: IBS
A new microscope with a reflective matrix and combinesincorporates the capabilities of both hardware and computational adaptive optics (AO). This technology was originally developed for ground-based astronomy to correct optical aberrations. A conventional confocal microscope measures the reflectance signal only at the focal point of illumination and rejects all out-of-focus light.
New microscope with reflective matrixdetects all scattered photons at positions other than the focal point. The scattered photons are then corrected computationally using a new CLASS (Closed Scattering Accumulation) algorithm. This AO algorithm uses all scattered light to selectively extract ballistic light and correct optical aberrations.
A reflective array microscope hasThe big advantage is that it can be directly combined with a conventional two-photon microscope, which is already widely used in the field of life sciences.
Our microscope allows you to explore the delicate internal structures deep in living tissues. This will greatly help in the early diagnosis of diseases and accelerate research in neuroscience.
Research Professor Yoon Seokchan
Read also
The Doomsday glacier turned out to be more dangerous than scientists thought. We tell the main thing
Found the alleged kingdom of the disappeared Hittites. What have archaeologists found?
An enzyme was discovered that reverses cell aging