For this particular cryopreservation method, oocytes are collected after castration or death of the animal, and after
This method allows you to store oocytes of rare andvaluable animals for an unlimited time, so that they can be used to obtain offspring using methods of assisted reproduction. This is necessary for further improvement, and the application of these methods to save endangered species. Ongoing research into African lions is an important step in this direction.
It is assumed that lion oocytes are verysensitive to cooling due to high lipid content, resulting in poor recovery after slow cooling. However, the vitrification method can solve this problem because the cells are frozen ultra-fast and in solutions with very high concentrations of cryoprotective agents.
Transport of new oocytes is oftencomplex and time-critical, while vitrification of oocytes makes it possible to transport them without restrictions. This way the oocytes can be fertilized later.
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