To increase sample concentration, previous teams used gel particles bound to
Of course, scientists have tried otherapproaches, but they all lack the ability to diagnose various viruses or other biological substances of interest, such as human chorionic gonadotropin (hCG), a hormone present in urine during pregnancy.
Researchers at the University of Cincinnati and Wright-Patterson Air Force Base tried two different devices based on similar membrane filter systems.
The first device was completelyan integrated concentration unit together with a component that could detect the virus in question. Using a vacuum, water was passed through the membrane, leaving the virus in a more concentrated solution, and the sample was analyzed on the same device.
"The time it takes to complete the processconcentration was very slow, ”said author Amy Drexelius. “On average, each device took about 30 minutes to complete the concentrating process. So we concluded that a vacuum-assisted automatic staging device was practically useless and turned to an alternative device using a much higher positive pressure. ”
The researchers realized that by applying highpressure instead of high vacuum, they can increase the pressure drop between the sample and the environment. Attached to their second device was a nitrogen cylinder and a regulator to generate pressures of up to 45 kg per square inch (6.4 cm).
The team examined saliva and urine samples with added protein samples from the influenza A virus. Other samples contained hCG.
The test results completely coincided with the seconddevice, showing the result of concentration 33 times higher than that of the original sample, scientists say. Concentration of a 1 ml sample can be achieved in five minutes, or even faster with a higher applied pressure.
The new coronavirus that causes COVID-19 was nottested, but this method should work for him as the technology can successfully detect influenza A. Both respiratory viruses are likely present in saliva. In addition, this method holds promise for pregnancy testing where early results are also highly desirable.
Read also
The Andromeda halo is approaching our galaxy. We tell you why it is important
Symptoms of coronavirus in children. What you should pay attention to?
It turned out that made the Mayan civilization leave their cities