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The team placed individual dye moleculesat well-defined distances from each other. This is accomplished using a new technique known as DNA origami. DNA as a storage medium in biology is used and programmed in such a way that molecules are arranged by folding DNA at will with dimensions of several nanometers.
Fluorescent light cannot be distinguished at firstindividual molecules on origami under a light microscope. Another trick is used to actually separate the molecules. Light from the origami structure passes through a semitransparent mirror and is recorded by photodetectors on both sides of the mirror.
It should be noted that a single molecule canemit only one light particle at a time, which is recorded by only one or the other detector, but not both. Considering the chronological order in which light hits the individual detectors, one can infer the exact number of dye molecules in the origami structure.
In this way, individual molecules can be counted.dye. The number of dye molecules is determined by DNA programming. An origami structure with one dye emits exactly one quantum of light - one with five emits exactly five.
Individual dye molecules are also respectivelyinteract with each other. When exposed to light, the dye absorbs energy. He can either emit it again as light, or pass it on to a nearby dye. However, if the neighboring dye is already in an excited state, two excitations will meet.
As with two cars tryingenter the same parking lot at the same time, the excitement disappears. Such annihilation is of great importance in molecular optoelectronics, such as organic light-emitting diodes or solar cells, but also plays a role in ultra-high resolution microscopy.
The research team was now able to showthat the nanoscopic interaction of dye molecules with each other can be directly traced by determining the arrival times of light particles on two light detectors. This approach offers a new method for ultrafast nanoscopy of molecular complexes that will also find applications in the life sciences.
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