Scientists create functional human blood cells using mice

Recently, research teams from the Guangzhou Institute of Biomedicine and Health Sciences (GIBH) of China

Academies of Sciences (CAS) have made new progress instudying cross-species chimerism with human pluripotent stem cells (hPSCs). They developed enhanced hPSCs, which for the first time allows the generation of functional human blood cells using cross-species chimerism technology.

Chimerism is a condition in whichGenetically different cells coexist in one organism. Many researchers are now exploring the possibility of using interspecies chimerism with hPSCs to produce functional human cells, tissues, or organs in large animals. Scientists hope this will solve the problem of shortage of donor tissues and organs. However, interspecies chimerism with hPSCs faces major obstacles due to the extremely low chimeric contribution of these important cells.

In a new study, scientists found thatrapid apoptosis of hPSCs in interspecies embryos was mainly due to growth deficiency and competition with host animal stem cells. The novel factor MYCN combined with the anti-apoptotic gene BCL2 effectively overcomes hPSC apoptosis and markedly promotes the formation of chimerism. 

As a result, scientists were able to isolate livinghuman blood progenitor cells from mice using interspecies chimerism with hPSCs for complementation. The resulting samples can be further cultured and differentiated into various blood cells.

Ultimately, improved hPSCs for cross-species chimerism will lead to advances in tissue and organ transplantation in the future, the scientists believe.

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