As gene editing technologies evolve with CRISPR, scientists increasingly need ways to
Doctors from the Brod Institute and Brigham Women's Hospital in a new study stated that they found molecules that can safely and quickly block the gene editing process using CRISPR.
According to the senior author of the new studyAmita Chodie, in similar works, other scientists are looking for CRISPR inhibitors among large proteins, while this group of doctors is focused on exploring the possibilities of using synthetic low-molecular objects.
As part of the study, scientists have collected a platformfor screening, which can accurately measure the activity of the CRISPR enzyme inside the cell. The neural network analyzed more than 15 thousand potential biological compounds and their reactions to CRISPR in order to identify the most effective ones for inhibition.
One connection - BRD0539 - turned out to be capableinhibit the Cas9 enzyme and untie it from the DNA. Its activity is highly dose dependent, which will allow in the future to control the degree of inhibition in almost live broadcast. In addition, BRD0539 was stable in plasma and easily removed from the blood.
Scientists note that while molecules cannot be used in clinical trials, because genetics have only begun to investigate the use of BRD0539 in the inhibition process.