The project uses the Perturb-seq approach to track the effect of turning on or off
When working with Perturb-seq technologyresearchers make genetic changes to cells using CRISPR-Cas9 and then use single-cell RNA sequencing to gather information about the RNA that is expressed as a result of that genetic change. The researchers note that RNA controls every aspect of cell behavior, so this method helps decipher most of the cellular effects of minimal genetic changes.
In a new study, scientists usedhuman blood cancer cell lines, as well as retinal-derived non-cancerous cells, to perform Perturb-seq on more than 2.5 million cells. Based on the data obtained, geneticists built a comprehensive map linking genotypes with phenotypes.
The authors believe that the results of the project will helpfuture research. For example, the creators of the integrated map themselves used the data to study the cellular effects of genes with unknown functions, to study the response of mitochondria to stress, and to screen for genes that cause chromosome loss or gain, a phenotype that has been difficult to study in the past.
“I think this dataset will allow all kinds of analyzes that we haven’t even thought of yet, to people who come from different areas of biology,” says Tom Norman, co-author of the study.
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